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KMID : 1101220030350020090
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2003 Volume.35 No. 2 p.90 ~ p.98
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Typing by Pulsed Field Gel Electrophoresis and Detection of Metallo-β-lactamase Gene Against Acinetobacter baumannii from Clinical Specimens
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Kim Shin-Moo
Kim Eun-Cheol
Choi Su-Youn
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Abstract
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We used modified Hodge and EDTA-disk synergy tests for the screening metallo-β-lactamase producing A. baumannii isolated from a lot of imipenem-resistant clinical strains during the period of 2002-2003 in partial University Hospital, Jeonbuk, Korea. We applied PCR to detect the blaIMP-1 and blaVIM-2 gene metallo-β -lactamase. Antimicrobial susceptibility testing was performed by disk diffusion and agar dilution methods according to National Committee for Clinical Laboratory Standards. Metallo-β-lactamase-producing A. baumannii by PCR were detected from 6 strains (7.6%) of 79 A. baumannii, while those were 1.3% in IMP-1 type and 6.3% in VIM-2 type. After DNA fingerprinting pattern of pulsed field gel electrophoresis (PFGE), 2 strains showed the same genotype. The MIC range of imipenem against VIM-2 type imipenem-resistant A. baumannii was 8-32 ㎍/ml, and MIC50 and MIC90 were 8 and 16 ㎍/ml. respectively, but that of imipenem against IMP-1 type A. baumanni was 2 ㎍/ml. To ciprofloxacin, 50% of the metallo-β-lactamase producing A. baumannii were resistant, while all were resistant to ampicillin, cefazolin, cefotaxime and cephalothin. It is concluded from this study that the modified Hodge and EDTA-disk synergy tests are simple tests for screening metallo-β-lactamase-producing A. baumannii, and IMP-1 and VIM-2 metallo-β-lactamase producing A. baumannii strains in Wonkwang University were rarely detected, thus, the PCR-aided rapid detection will be helpful for the early recognition of emerging blaIMP-1 and blaVIM-2 gene positive clinical isolates which demonstrate consistent resistance to carbapenm, and the PFGE method is useful for epidemiological studies of A. baumannii strains
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KEYWORD
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Metallo-β-lactamase, blaIMP-1, blaVIM-2, Imipenem-resistant A. baumannii, MIC
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